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E. coli NEB5-alpha · DH5α derivative
DNA-binding transcriptional repressor LacI
The published complete genome represents NEB 5-alpha, a named derivative of DH5α.
Do not treat this derivative as an identical reference for every DH5α stock.
Complete Genome Sequence of NEB 5-alpha, a Derivative of E. coli K-12 DH5α
Observed outcome · Related strain
The study removed lacI from MG1655 to obtain lacZ expression without adding an inducer.
Study strain: MG1655 derivative with lacI deleted
Conditions: Reporter-assay strain construction; this does not quantify expression in every medium.
A High-Throughput Approach To Identify Compounds That Impair Envelope Integrity in Escherichia coli
Gene function · Related strain
LacI binds DNA to repress transcription of genes involved in lactose transport and use.
Study strain: K-12 MG1655
Conditions: Expression also depends on promoter context and other regulators.
Compare the experimental backgrounds and read the supporting sources.
NCBI reference annotation · Reference gene interval · Download gene DNA (FASTA)
Gene-level evidence does not identify a validated nucleotide edit. Evaluate the study’s strain and conditions before applying an observation to another background.