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Public evidence
Escherichia colilacZlacI

Can I reuse an MG1655 gene location or phenotype in BW25113 or DH5α?

Change

Pre-existing strain alleles; no new edit is proposed.

Measured

Genome sequence and annotated structural differences.

Observed outcome

BW25113 already replaces part of lacZ with tandem rrnB terminators. Its reported lacI allele is lacI+, correcting an earlier lacIq description.

Source 1
System
Escherichia coli · BW25113, DH5α KAIST stock, and NEB 5-alpha
Setting
Independent sequencing reports and a deposited draft assembly.
Comparison
Named deposited stocks; BW25113 was compared with MG1655.

What was observed

Checked findings go into your research brief.

3
ObservedSource 1

BW25113 already replaces part of lacZ with tandem rrnB terminators. Its reported lacI allele is lacI+, correcting an earlier lacIq description.

Genome Announcement, sequence-comparison paragraph

ObservedSource 2

NEB 5-alpha carries the alpha-complementable ΔlacZ58(M15) allele in a phage-derived insertion; its ordinary lac region is deleted. It is a named DH5α derivative.

Genome Announcement, paragraphs describing the finished genome and genotype

ObservedSource 3

The KAIST DH5alpha reference is an 89-contig assembly, so locations belong to named contigs.

Assembly statistics and Sample details

Measurement details
Reported value
89 contigs
Group
GCF_000982435.1

What this supports

Interpretation
  • Reference choice must precede interpretation of a locus or phenotype.
What this does not establish
  • A DH5α label does not establish sequence identity to NEB 5-alpha or another deposited stock.
Gene location & edit specificity

No exact edit interval established by this evidence review.

BW25113 reference DH5α · KAIST draft NEB 5-alpha reference

Same gene, different setting

Compare the contexts before carrying a finding across them.

Does deleting lacZ stop growth?Escherichia coli · MG1655-derived NCM3384 and NCM3387
A weaker reporter can mean less substrate accessEscherichia coli · MG1655 ΔlacI versus MG1655 ΔlacI ΔlacY

Sources

Reviewed Sep 26, 2026
1Complete Genome Sequence of Escherichia coli BW251132014 · Peer-reviewed genome report
Reviewed
Full text
Sections
Genome Announcement · Nucleotide sequence accession number
DOI
10.1128/genomeA.01038-14
replacement of a section of lacZ with four tandem rrnB terminators
Genome Announcement, sequence-comparison paragraph
Read the source
2Complete Genome Sequence of NEB 5-alpha, a Derivative of Escherichia coli K-12 DH5α2016 · Peer-reviewed genome report
Reviewed
Full text
Sections
Genome Announcement · Accession number(s)
DOI
10.1128/genomeA.01245-16
Read the source
3NCBI assembly ASM98243v1: DH5alpha laboratory stock2015 · Reference assembly
Reviewed
Reference record
Sections
Assembly statistics · Sample details
Read the source