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E. coli NEB5-alpha · DH5α derivative
lactose permease
The published complete genome represents NEB 5-alpha, a named derivative of DH5α.
Do not treat this derivative as an identical reference for every DH5α stock.
Complete Genome Sequence of NEB 5-alpha, a Derivative of E. coli K-12 DH5α
Observed outcome · Related strain
Removing lacY from an MG1655 lacI-deletion background lowered intact-cell ONPG hydrolysis. Substrate transport changed the readout even though lacZ remained.
Study strain: MG1655 derivative with lacI and lacY deleted
Conditions: Envelope-integrity reporter assay; ONPG access to intracellular beta-galactosidase is the relevant measurement.
A High-Throughput Approach To Identify Compounds That Impair Envelope Integrity in Escherichia coli
Gene function · Related strain
LacY is a lactose/proton symporter that brings lactose and other galactosides into the cell.
Study strain: K-12 MG1655
Conditions: Transport is distinct from intracellular substrate breakdown.
NCBI reference annotation · Reference gene interval · Download gene DNA (FASTA)
Gene-level evidence does not identify a validated nucleotide edit. Evaluate the study’s strain and conditions before applying an observation to another background.