ZipHelixDH5α · KAIST draft
This DH5α laboratory stock was assembled into 89 contigs. Gene positions belong to the named contig.
It is not a complete chromosome or a verified match to every commercial DH5α stock.
E. coli DH5α · KAIST draft
lacI
DNA-binding transcriptional repressor LacI
- Locus tag
- NJ74_RS08955
- Reference assembly
- GCF_000982435.1
- Sequence
- NZ_JRYM01000017.1
- Gene interval
- 35,488–36,570 · - strand
- Coordinate system
- 1-based, inclusive
Reference context
Published evidence
Observed outcome · Related strain
lacI deletion permits constitutive lacZ expression
The study removed lacI from MG1655 to obtain lacZ expression without adding an inducer.
Study strain: MG1655 derivative with lacI deleted
Conditions: Reporter-assay strain construction; this does not quantify expression in every medium.
A High-Throughput Approach To Identify Compounds That Impair Envelope Integrity in Escherichia coli
Gene function · Related strain
Lac operon repression
LacI binds DNA to repress transcription of genes involved in lactose transport and use.
Study strain: K-12 MG1655
Conditions: Expression also depends on promoter context and other regulators.
Review the experimental evidence
Compare the experimental backgrounds and read the supporting sources.
- Can I reuse an MG1655 gene location or phenotype in BW25113 or DH5α? — BW25113, DH5α KAIST stock, and NEB 5-alpha
Nearby genes
- lacA · NJ74_RS08940 — galactoside O-acetyltransferase
- lacY · NJ74_RS08945 — lactose permease
- lacZ · NJ74_RS08950 — beta-galactosidase
- lacI · NJ74_RS08955 — DNA-binding transcriptional repressor LacI
- mhpR · NJ74_RS08960 — DNA-binding transcriptional regulator
- mhpA · NJ74_RS08965 — bifunctional 3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase
- mhpB · NJ74_RS08970 — 2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase
Reference and sequence
NCBI reference annotation · Reference gene interval · Download gene DNA (FASTA)
Gene-level evidence does not identify a validated nucleotide edit. Evaluate the study’s strain and conditions before applying an observation to another background.