ZipHelixDH5α · KAIST draft
This DH5α laboratory stock was assembled into 89 contigs. Gene positions belong to the named contig.
It is not a complete chromosome or a verified match to every commercial DH5α stock.
E. coli DH5α · KAIST draft
lacY
lactose permease
- Locus tag
- NJ74_RS08945
- Reference assembly
- GCF_000982435.1
- Sequence
- NZ_JRYM01000017.1
- Gene interval
- 31,079–32,332 · - strand
- Coordinate system
- 1-based, inclusive
Reference context
Published evidence
Observed outcome · Related strain
lacY deletion lowers the ONPG readout
Removing lacY from an MG1655 lacI-deletion background lowered intact-cell ONPG hydrolysis. Substrate transport changed the readout even though lacZ remained.
Study strain: MG1655 derivative with lacI and lacY deleted
Conditions: Envelope-integrity reporter assay; ONPG access to intracellular beta-galactosidase is the relevant measurement.
A High-Throughput Approach To Identify Compounds That Impair Envelope Integrity in Escherichia coli
Gene function · Related strain
Lactose transport
LacY is a lactose/proton symporter that brings lactose and other galactosides into the cell.
Study strain: K-12 MG1655
Conditions: Transport is distinct from intracellular substrate breakdown.
Nearby genes
- cynS · NJ74_RS25655 — cyanase
- cynX · NJ74_RS08935 — cyanate transporter CynX
- lacA · NJ74_RS08940 — galactoside O-acetyltransferase
- lacY · NJ74_RS08945 — lactose permease
- lacZ · NJ74_RS08950 — beta-galactosidase
- lacI · NJ74_RS08955 — DNA-binding transcriptional repressor LacI
- mhpR · NJ74_RS08960 — DNA-binding transcriptional regulator
Reference and sequence
NCBI reference annotation · Reference gene interval · Download gene DNA (FASTA)
Gene-level evidence does not identify a validated nucleotide edit. Evaluate the study’s strain and conditions before applying an observation to another background.