E. coli BL21(DE3)

lacI

DNA-binding transcriptional repressor LacI

Locus tag: ECD_RS01580
Reference assembly: GCF_000022665.1
Sequence: NC_012971.2
Gene interval: 335,963–337,045 · - strand
Coordinate system: 1-based, inclusive

Published evidence

Observed outcome · Related strain

lacI deletion permits constitutive lacZ expression

The study removed lacI from MG1655 to obtain lacZ expression without adding an inducer.

Study strain: MG1655 derivative with lacI deleted

Conditions: Reporter-assay strain construction; this does not quantify expression in every medium.

A High-Throughput Approach To Identify Compounds That Impair Envelope Integrity in Escherichia coli

Gene function · Related strain

Lac operon repression

LacI binds DNA to repress transcription of genes involved in lactose transport and use.

Study strain: K-12 MG1655

Conditions: Expression also depends on promoter context and other regulators.

NCBI Gene: lacI (b0345)

Nearby genes

lacA · ECD_RS01565 — galactoside O-acetyltransferase
lacY · ECD_RS01570 — lactose permease
lacZ · ECD_RS01575 — beta-galactosidase
lacI · ECD_RS01580 — DNA-binding transcriptional repressor LacI
mhpR · ECD_RS01585 — DNA-binding transcriptional regulator
mhpA · ECD_RS01590 — bifunctional 3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase
mhpB · ECD_RS01595 — 2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase

Reference and sequence

NCBI reference annotation · Reference gene interval · Download gene DNA (FASTA)

Gene-level evidence does not identify a validated nucleotide edit. Evaluate the study’s strain and conditions before applying an observation to another background.