E. coli BL21(DE3)

lacZ

beta-galactosidase

Locus tag: ECD_RS01575
Reference assembly: GCF_000022665.1
Sequence: NC_012971.2
Gene interval: 332,766–335,840 · - strand
Coordinate system: 1-based, inclusive

Published evidence

Observed outcome · Related strain

lacZ deletion did not slow growth on galactose in this comparison

Table 5 reports the same galactose doubling time for the study wild type and its lacZ-deletion derivative. A lacY amber mutant grew more slowly.

Study strain: MG1655-derived NCM3387 compared with NCM3384

Conditions: Defined medium with galactose and IPTG; this is not a lactose-only growth result.

Physiological Studies of Escherichia coli Strain MG1655: Growth Defects and Apparent Cross-Regulation of Gene Expression

Gene function · Related strain

Lactose breakdown

lacZ encodes beta-galactosidase. Its enzyme activity supports lactose breakdown and chromogenic beta-galactosidase assays.

Study strain: K-12 MG1655

Conditions: A color assay requires a suitable substrate; expression and substrate access also matter.

NCBI Gene: lacZ (b0344)

Nearby genes

cynX · ECD_RS01560 — cyanate transporter CynX
lacA · ECD_RS01565 — galactoside O-acetyltransferase
lacY · ECD_RS01570 — lactose permease
lacZ · ECD_RS01575 — beta-galactosidase
lacI · ECD_RS01580 — DNA-binding transcriptional repressor LacI
mhpR · ECD_RS01585 — DNA-binding transcriptional regulator
mhpA · ECD_RS01590 — bifunctional 3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase

Reference and sequence

NCBI reference annotation · Reference gene interval · Download gene DNA (FASTA)

Gene-level evidence does not identify a validated nucleotide edit. Evaluate the study’s strain and conditions before applying an observation to another background.