E. coli BL21(DE3)

lacY

lactose permease

Locus tag: ECD_RS01570
Reference assembly: GCF_000022665.1
Sequence: NC_012971.2
Gene interval: 331,461–332,714 · - strand
Coordinate system: 1-based, inclusive

Published evidence

Observed outcome · Related strain

lacY deletion lowers the ONPG readout

Removing lacY from an MG1655 lacI-deletion background lowered intact-cell ONPG hydrolysis. Substrate transport changed the readout even though lacZ remained.

Study strain: MG1655 derivative with lacI and lacY deleted

Conditions: Envelope-integrity reporter assay; ONPG access to intracellular beta-galactosidase is the relevant measurement.

A High-Throughput Approach To Identify Compounds That Impair Envelope Integrity in Escherichia coli

Gene function · Related strain

Lactose transport

LacY is a lactose/proton symporter that brings lactose and other galactosides into the cell.

Study strain: K-12 MG1655

Conditions: Transport is distinct from intracellular substrate breakdown.

NCBI Gene: lacY (b0343)

Nearby genes

cynS · ECD_RS01555 — cyanase
cynX · ECD_RS01560 — cyanate transporter CynX
lacA · ECD_RS01565 — galactoside O-acetyltransferase
lacY · ECD_RS01570 — lactose permease
lacZ · ECD_RS01575 — beta-galactosidase
lacI · ECD_RS01580 — DNA-binding transcriptional repressor LacI
mhpR · ECD_RS01585 — DNA-binding transcriptional regulator

Reference and sequence

NCBI reference annotation · Reference gene interval · Download gene DNA (FASTA)

Gene-level evidence does not identify a validated nucleotide edit. Evaluate the study’s strain and conditions before applying an observation to another background.