ZipHelixDH5α · ANSES stock
This complete chromosome represents the DH5α laboratory stock submitted by ANSES Ploufragan.
The accession identifies one sequenced stock; other DH5α stocks may differ.
E. coli DH5α · ANSES stock
lacI
DNA-binding transcriptional repressor LacI
- Locus tag
- CDN75_RS00980
- Reference assembly
- GCF_002848225.1
- Sequence
- NZ_CP025520.1
- Gene interval
- 153,918–155,000 · - strand
- Coordinate system
- 1-based, inclusive
Reference context
Published evidence
Observed outcome · Related strain
lacI deletion permits constitutive lacZ expression
The study removed lacI from MG1655 to obtain lacZ expression without adding an inducer.
Study strain: MG1655 derivative with lacI deleted
Conditions: Reporter-assay strain construction; this does not quantify expression in every medium.
A High-Throughput Approach To Identify Compounds That Impair Envelope Integrity in Escherichia coli
Gene function · Related strain
Lac operon repression
LacI binds DNA to repress transcription of genes involved in lactose transport and use.
Study strain: K-12 MG1655
Conditions: Expression also depends on promoter context and other regulators.
Nearby genes
- lacA · CDN75_RS00960 — galactoside O-acetyltransferase
- lacY · CDN75_RS00965 — lactose permease
- lacZ · CDN75_RS00970 — beta-galactosidase
- lacI · CDN75_RS00980 — DNA-binding transcriptional repressor LacI
- mhpR · CDN75_RS00985 — DNA-binding transcriptional regulator
- mhpA · CDN75_RS00990 — bifunctional 3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase
- mhpB · CDN75_RS00995 — 2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase
Reference and sequence
NCBI reference annotation · Reference gene interval · Download gene DNA (FASTA)
Gene-level evidence does not identify a validated nucleotide edit. Evaluate the study’s strain and conditions before applying an observation to another background.