ZipHelixDH5α · ANSES stock
This complete chromosome represents the DH5α laboratory stock submitted by ANSES Ploufragan.
The accession identifies one sequenced stock; other DH5α stocks may differ.
E. coli DH5α · ANSES stock
lacY
lactose permease
- Locus tag
- CDN75_RS00965
- Reference assembly
- GCF_002848225.1
- Sequence
- NZ_CP025520.1
- Gene interval
- 149,509–150,762 · - strand
- Coordinate system
- 1-based, inclusive
Reference context
Published evidence
Observed outcome · Related strain
lacY deletion lowers the ONPG readout
Removing lacY from an MG1655 lacI-deletion background lowered intact-cell ONPG hydrolysis. Substrate transport changed the readout even though lacZ remained.
Study strain: MG1655 derivative with lacI and lacY deleted
Conditions: Envelope-integrity reporter assay; ONPG access to intracellular beta-galactosidase is the relevant measurement.
A High-Throughput Approach To Identify Compounds That Impair Envelope Integrity in Escherichia coli
Gene function · Related strain
Lactose transport
LacY is a lactose/proton symporter that brings lactose and other galactosides into the cell.
Study strain: K-12 MG1655
Conditions: Transport is distinct from intracellular substrate breakdown.
Nearby genes
- cynS · CDN75_RS24480 — cyanase
- cynX · CDN75_RS00955 — cyanate transporter CynX
- lacA · CDN75_RS00960 — galactoside O-acetyltransferase
- lacY · CDN75_RS00965 — lactose permease
- lacZ · CDN75_RS00970 — beta-galactosidase
- lacI · CDN75_RS00980 — DNA-binding transcriptional repressor LacI
- mhpR · CDN75_RS00985 — DNA-binding transcriptional regulator
Reference and sequence
NCBI reference annotation · Reference gene interval · Download gene DNA (FASTA)
Gene-level evidence does not identify a validated nucleotide edit. Evaluate the study’s strain and conditions before applying an observation to another background.