E. coli K-12 BW25113

lacI

DNA-binding transcriptional repressor LacI

Locus tag: BW25113_RS01775
Reference assembly: GCF_000750555.1
Sequence: NZ_CP009273.1
Gene interval: 360,661–361,743 · - strand
Coordinate system: 1-based, inclusive

Published evidence

Observed outcome · Related strain

lacI deletion permits constitutive lacZ expression

The study removed lacI from MG1655 to obtain lacZ expression without adding an inducer.

Study strain: MG1655 derivative with lacI deleted

Conditions: Reporter-assay strain construction; this does not quantify expression in every medium.

A High-Throughput Approach To Identify Compounds That Impair Envelope Integrity in Escherichia coli

Gene function · Related strain

Lac operon repression

LacI binds DNA to repress transcription of genes involved in lactose transport and use.

Study strain: K-12 MG1655

Conditions: Expression also depends on promoter context and other regulators.

NCBI Gene: lacI (b0345)

Review the experimental evidence

Compare the experimental backgrounds and read the supporting sources.

Can I reuse an MG1655 gene location or phenotype in BW25113 or DH5α? — BW25113, DH5α KAIST stock, and NEB 5-alpha

Nearby genes

lacY · BW25113_RS01760 — lactose permease
BW25113_RS01765 · BW25113_RS01765 — beta-galactosidase domain 4-containing protein
BW25113_RS23320 · BW25113_RS23320 — relaxase/mobilization nuclease domain-containing protein
lacI · BW25113_RS01775 — DNA-binding transcriptional repressor LacI
mhpR · BW25113_RS01780 — DNA-binding transcriptional regulator
mhpA · BW25113_RS01785 — bifunctional 3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase
mhpB · BW25113_RS01790 — 2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase

Reference and sequence

NCBI reference annotation · Reference gene interval · Download gene DNA (FASTA)

Gene-level evidence does not identify a validated nucleotide edit. Evaluate the study’s strain and conditions before applying an observation to another background.