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E. coli NEB5-alpha · DH5α derivative
histidinol dehydrogenase
The published complete genome represents NEB 5-alpha, a named derivative of DH5α.
Do not treat this derivative as an identical reference for every DH5α stock.
Complete Genome Sequence of NEB 5-alpha, a Derivative of E. coli K-12 DH5α
Observed outcome · Related strain
The BW25113-derived hisD deletion strain JW2002 was used as a histidine auxotroph. In the study, its growth readout increased with supplied histidine.
Study strain: BW25113-derived JW2002 with an introduced EGFP reporter
Conditions: Minimal-medium biosensor assay. An introduced EGFP reporter was used alongside turbidity; fluorescence was not a native hisD product.
Design and Characterization of Auxotrophy-Based Amino Acid Biosensors
Gene function · Related strain
HisD catalyzes the last two steps of histidine biosynthesis.
Study strain: K-12 MG1655
Conditions: Histidine supplied by medium can mask a biosynthetic defect.
NCBI reference annotation · Reference gene interval · Download gene DNA (FASTA)
Gene-level evidence does not identify a validated nucleotide edit. Evaluate the study’s strain and conditions before applying an observation to another background.