E. coli NEB5-alpha · DH5α derivative

araA

L-arabinose isomerase

Locus tag: NEB5A_RS00315
Reference assembly: GCF_001723505.1
Sequence: NZ_CP017100.1
Gene interval: 66,835–68,337 · - strand
Coordinate system: 1-based, inclusive

Reference context

NEB 5-alpha · DH5α derivative

The published complete genome represents NEB 5-alpha, a named derivative of DH5α.

Do not treat this derivative as an identical reference for every DH5α stock.

Complete Genome Sequence of NEB 5-alpha, a Derivative of E. coli K-12 DH5α

Published evidence

Gene function · Related strain

Arabinose metabolism

AraA converts L-arabinose to L-ribulose, the first enzymatic step of its degradation pathway.

Study strain: K-12 MG1655

Conditions: Function annotation does not establish the effect of an arbitrary base substitution. BW25113 already lacks araBAD.

NCBI Gene: araA (b0062)

Nearby genes

rapA · NEB5A_RS00300 — RNA polymerase-associated protein RapA
polB · NEB5A_RS00305 — DNA polymerase II
araD · NEB5A_RS00310 — L-ribulose-5-phosphate 4-epimerase
araA · NEB5A_RS00315 — L-arabinose isomerase
araB · NEB5A_RS00320 — ribulokinase
araC · NEB5A_RS00325 — arabinose operon transcriptional regulator AraC
yabI · NEB5A_RS00330 — DedA family protein

Reference and sequence

NCBI reference annotation · Reference gene interval · Download gene DNA (FASTA)

Gene-level evidence does not identify a validated nucleotide edit. Evaluate the study’s strain and conditions before applying an observation to another background.