E. coli K-12 MG1655

hisD

histidinal/histidinol dehydrogenase

Locus tag: b2020
Reference assembly: GCF_000005845.2
Sequence: NC_000913.3
Gene interval: 2,091,097–2,092,401 · + strand
Coordinate system: 1-based, inclusive

Published evidence

Gene function · Same reference background

Histidine biosynthesis

HisD catalyzes the last two steps of histidine biosynthesis.

Study strain: K-12 MG1655

Conditions: Histidine supplied by medium can mask a biosynthetic defect.

NCBI Gene: hisD (b2020)

Observed outcome · Related strain

hisD deletion makes growth depend on supplied histidine

The BW25113-derived hisD deletion strain JW2002 was used as a histidine auxotroph. In the study, its growth readout increased with supplied histidine.

Study strain: BW25113-derived JW2002 with an introduced EGFP reporter

Conditions: Minimal-medium biosensor assay. An introduced EGFP reporter was used alongside turbidity; fluorescence was not a native hisD product.

Design and Characterization of Auxotrophy-Based Amino Acid Biosensors

Nearby genes

yefM · b2017 — YefM antitoxin of the YoeB-YefM toxin-antitoxin pair and DNA binding transcriptional repressor
hisL · b2018 — his operon leader peptide
hisG · b2019 — ATP phosphoribosyltransferase
hisD · b2020 — histidinal/histidinol dehydrogenase
hisC · b2021 — histidinol-phosphate aminotransferase
hisB · b2022 — imidazoleglycerol-phosphate dehydratase/histidinol-phosphatase
hisH · b2023 — imidazole glycerol phosphate synthase subunit HisH

Reference and sequence

NCBI reference annotation · Reference gene interval · Download gene DNA (FASTA)

Gene-level evidence does not identify a validated nucleotide edit. Evaluate the study’s strain and conditions before applying an observation to another background.