E. coli K-12 MG1655

lacY

lactose permease

Locus tag: b0343
Reference assembly: GCF_000005845.2
Sequence: NC_000913.3
Gene interval: 361,926–363,179 · - strand
Coordinate system: 1-based, inclusive

Published evidence

Observed outcome · Same reference background

lacY deletion lowers the ONPG readout

Removing lacY from an MG1655 lacI-deletion background lowered intact-cell ONPG hydrolysis. Substrate transport changed the readout even though lacZ remained.

Study strain: MG1655 derivative with lacI and lacY deleted

Conditions: Envelope-integrity reporter assay; ONPG access to intracellular beta-galactosidase is the relevant measurement.

A High-Throughput Approach To Identify Compounds That Impair Envelope Integrity in Escherichia coli

Gene function · Same reference background

Lactose transport

LacY is a lactose/proton symporter that brings lactose and other galactosides into the cell.

Study strain: K-12 MG1655

Conditions: Transport is distinct from intracellular substrate breakdown.

NCBI Gene: lacY (b0343)

Review the experimental evidence

Compare the experimental backgrounds and read the supporting sources.

Does a lacZ deletion prevent E. coli growth, or does the carbon source change the conclusion? — MG1655-derived NCM3384 and NCM3387
Does lower ONPG color mean less LacZ enzyme? — MG1655 ΔlacI versus MG1655 ΔlacI ΔlacY

Nearby genes

cynS · b0340 — cyanase
cynX · b0341 — cyanate transporter
lacA · b0342 — galactoside O-acetyltransferase
lacY · b0343 — lactose permease
lacZ · b0344 — beta-galactosidase
lacI · b0345 — DNA-binding transcriptional repressor LacI
mhpR · b0346 — DNA-binding transcriptional activator MhpR

Reference and sequence

NCBI reference annotation · Reference gene interval · Download gene DNA (FASTA)

Gene-level evidence does not identify a validated nucleotide edit. Evaluate the study’s strain and conditions before applying an observation to another background.