ZipHelixDH5α · KAIST draft
This DH5α laboratory stock was assembled into 89 contigs. Gene positions belong to the named contig.
It is not a complete chromosome or a verified match to every commercial DH5α stock.
E. coli DH5α · KAIST draft
hisD
histidinol dehydrogenase
- Locus tag
- NJ74_RS14430
- Reference assembly
- GCF_000982435.1
- Sequence
- NZ_JRYM01000029.1
- Gene interval
- 9,367–10,671 · - strand
- Coordinate system
- 1-based, inclusive
Reference context
Published evidence
Observed outcome · Related strain
hisD deletion makes growth depend on supplied histidine
The BW25113-derived hisD deletion strain JW2002 was used as a histidine auxotroph. In the study, its growth readout increased with supplied histidine.
Study strain: BW25113-derived JW2002 with an introduced EGFP reporter
Conditions: Minimal-medium biosensor assay. An introduced EGFP reporter was used alongside turbidity; fluorescence was not a native hisD product.
Design and Characterization of Auxotrophy-Based Amino Acid Biosensors
Gene function · Related strain
Histidine biosynthesis
HisD catalyzes the last two steps of histidine biosynthesis.
Study strain: K-12 MG1655
Conditions: Histidine supplied by medium can mask a biosynthetic defect.
Nearby genes
- hisH · NJ74_RS14415 — imidazole glycerol phosphate synthase subunit HisH
- hisB · NJ74_RS14420 — bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB
- hisC · NJ74_RS14425 — histidinol-phosphate transaminase
- hisD · NJ74_RS14430 — histidinol dehydrogenase
- hisG · NJ74_RS14435 — ATP phosphoribosyltransferase
- hisL · NJ74_RS23770 — his operon leader peptide
- yefM · NJ74_RS14440 — YoeB-YefM toxin-antitoxin system antitoxin YefM
Reference and sequence
NCBI reference annotation · Reference gene interval · Download gene DNA (FASTA)
Gene-level evidence does not identify a validated nucleotide edit. Evaluate the study’s strain and conditions before applying an observation to another background.