ZipHelixDH5α · ANSES stock
This complete chromosome represents the DH5α laboratory stock submitted by ANSES Ploufragan.
The accession identifies one sequenced stock; other DH5α stocks may differ.
E. coli DH5α · ANSES stock
hisD
histidinol dehydrogenase
- Locus tag
- CDN75_RS05280
- Reference assembly
- GCF_002848225.1
- Sequence
- NZ_CP025520.1
- Gene interval
- 948,960–950,264 · + strand
- Coordinate system
- 1-based, inclusive
Reference context
Published evidence
Observed outcome · Related strain
hisD deletion makes growth depend on supplied histidine
The BW25113-derived hisD deletion strain JW2002 was used as a histidine auxotroph. In the study, its growth readout increased with supplied histidine.
Study strain: BW25113-derived JW2002 with an introduced EGFP reporter
Conditions: Minimal-medium biosensor assay. An introduced EGFP reporter was used alongside turbidity; fluorescence was not a native hisD product.
Design and Characterization of Auxotrophy-Based Amino Acid Biosensors
Gene function · Related strain
Histidine biosynthesis
HisD catalyzes the last two steps of histidine biosynthesis.
Study strain: K-12 MG1655
Conditions: Histidine supplied by medium can mask a biosynthetic defect.
Nearby genes
- yefM · CDN75_RS05260 — YoeB-YefM toxin-antitoxin system antitoxin YefM
- hisL · CDN75_RS05265 — his operon leader peptide
- hisG · CDN75_RS05275 — ATP phosphoribosyltransferase
- hisD · CDN75_RS05280 — histidinol dehydrogenase
- hisC · CDN75_RS05285 — histidinol-phosphate transaminase
- hisB · CDN75_RS05290 — bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB
- hisH · CDN75_RS05295 — imidazole glycerol phosphate synthase subunit HisH
Reference and sequence
NCBI reference annotation · Reference gene interval · Download gene DNA (FASTA)
Gene-level evidence does not identify a validated nucleotide edit. Evaluate the study’s strain and conditions before applying an observation to another background.