Reference genomes / E. coli BL21(DE3) E. coli BL21(DE3)
hisD histidinol dehydrogenase
Locus tag ECD_RS10140 Reference assembly GCF_000022665.1 Sequence NC_012971.2 Gene interval 1,986,900–1,988,204 · + strand Coordinate system 1-based, inclusive Published evidence Observed outcome · Related strain
hisD deletion makes growth depend on supplied histidine The BW25113-derived hisD deletion strain JW2002 was used as a histidine auxotroph. In the study, its growth readout increased with supplied histidine.
Study strain: BW25113-derived JW2002 with an introduced EGFP reporter
Conditions: Minimal-medium biosensor assay. An introduced EGFP reporter was used alongside turbidity; fluorescence was not a native hisD product.
Design and Characterization of Auxotrophy-Based Amino Acid Biosensors
Gene function · Related strain
Histidine biosynthesis HisD catalyzes the last two steps of histidine biosynthesis.
Study strain: K-12 MG1655
Conditions: Histidine supplied by medium can mask a biosynthetic defect.
NCBI Gene: hisD (b2020)
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