E. coli BL21(DE3)

lacI

DNA-binding transcriptional repressor LacI

Locus tag: ECD_RS03700
Reference assembly: GCF_000022665.1
Sequence: NC_012971.2
Gene interval: 748,736–749,818 · + strand
Coordinate system: 1-based, inclusive

Published evidence

Observed outcome · Related strain

lacI deletion permits constitutive lacZ expression

The study removed lacI from MG1655 to obtain lacZ expression without adding an inducer.

Study strain: MG1655 derivative with lacI deleted

Conditions: Reporter-assay strain construction; this does not quantify expression in every medium.

A High-Throughput Approach To Identify Compounds That Impair Envelope Integrity in Escherichia coli

Gene function · Related strain

Lac operon repression

LacI binds DNA to repress transcription of genes involved in lactose transport and use.

Study strain: K-12 MG1655

Conditions: Expression also depends on promoter context and other regulators.

NCBI Gene: lacI (b0345)

Nearby genes

ECD_RS26515 · ECD_RS26515 — hypothetical protein
ybhC · ECD_RS03695 — putative acyl-CoA thioester hydrolase
ECD_RS23070 · ECD_RS23070 — tyrosine-type recombinase/integrase
lacI · ECD_RS03700 — DNA-binding transcriptional repressor LacI
ECD_RS03705 · ECD_RS03705 — sugar-binding domain-containing protein
ECD_RS03710 · ECD_RS03710 — DNA-directed RNA polymerase
ECD_RS03715 · ECD_RS03715 — phage integrase Arm DNA-binding domain-containing protein

Reference and sequence

NCBI reference annotation · Reference gene interval · Download gene DNA (FASTA)

Gene-level evidence does not identify a validated nucleotide edit. Evaluate the study’s strain and conditions before applying an observation to another background.